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Part:BBa_K1844004

Designed by: David Lips   Group: iGEM15_Amsterdam   (2015-09-17)


Arg-H KO Construct

This part includes a construct to knock-out the Arg-H gene in Synechocystis PCC. 6803, which leads to an arginine auxotrophy in the mutant Synechocystis strain. It can be used to create a dependency on an arginine producer in a synthetic consortium of multiple species.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 959
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 959
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 959
    Illegal NgoMIV site found at 257
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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